CD300a ELISA 试剂盒
CD300A
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
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- 抗原 See all CD300a (CD300A) ELISA试剂盒
- CD300a (CD300A)
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human CD300a (LMIR1) SpeedELISA Kit.
- 品牌
- SpeedELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Compressed workflow with only one wash step and three incubation steps
- 3 hours total processing time
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Streptavidin-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Capture Antibody
- HRP Conjugated Detection Antibody
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product CD300A ELISA Kit
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CD300a (CD300A) ELISA Kit
CD300A 适用: 人 Colorimetric Sandwich ELISA 0.313-20 ng/mL Cell Culture Cells, Plasma, Serum, Urine
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- 样本量
- 100 μL
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 70 μL of the Antibody Cocktail to each well.
- Incubate 10 minutes at RT.
- Add 50 μL of sample or standard to each well.
- Incubate 2 h at RT.
- Wash Wells
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
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May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles. - 有效期
- 6 months
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- 抗原 See all CD300a (CD300A) ELISA试剂盒
- CD300a (CD300A)
- 别名
- CD300a (CD300A 产品)
- 别名
- CD300L-B1 ELISA Kit, CD300A ELISA Kit, clm8 ELISA Kit, B230315M08Rik ELISA Kit, Clm8 ELISA Kit, LMIR1 ELISA Kit, MAIR-I ELISA Kit, MAIR-Ia ELISA Kit, MMAC8 ELISA Kit, Pigr4 ELISA Kit, mcpir1 ELISA Kit, CLM-8 ELISA Kit, CMRF-35-H9 ELISA Kit, CMRF-35H ELISA Kit, CMRF35-H ELISA Kit, CMRF35-H9 ELISA Kit, CMRF35H ELISA Kit, CMRF35H9 ELISA Kit, IGSF12 ELISA Kit, IRC1 ELISA Kit, IRC1/IRC2 ELISA Kit, IRC2 ELISA Kit, IRp60 ELISA Kit, CD300 molecule like family member g ELISA Kit, CD300a molecule ELISA Kit, CMRF35-like molecule 8 ELISA Kit, CD300A molecule ELISA Kit, Cd300a molecule ELISA Kit, CD300LG ELISA Kit, CD300A ELISA Kit, LOC483289 ELISA Kit, clm8 ELISA Kit, Cd300a ELISA Kit
- 基因ID
- 11314
- UniProt
- Q9UGN4
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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