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ZAP70 ELISA 试剂盒

ZAP70 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
产品编号 ABIN4885072
发货至: 中国
  • 抗原 See all ZAP70 ELISA试剂盒
    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
    适用
    • 9
    • 5
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Human ZAP70 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes .
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all ZAP70 ELISA试剂盒
    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
    别名
    ZAP70 (ZAP70 产品)
    别名
    SRK ELISA Kit, STCD ELISA Kit, STD ELISA Kit, TZK ELISA Kit, ZAP-70 ELISA Kit, 70kDa ELISA Kit, AI327364 ELISA Kit, Srk ELISA Kit, mrtle ELISA Kit, mur ELISA Kit, zap70 ELISA Kit, MGC80670 ELISA Kit, MGC88962 ELISA Kit, ZAP70 ELISA Kit, zgc:110383 ELISA Kit, zeta chain of T-cell receptor associated protein kinase 70 ELISA Kit, putative tyrosine-protein kinase zap-70 ELISA Kit, zeta-chain (TCR) associated protein kinase ELISA Kit, zeta chain of T cell receptor associated protein kinase 70 ELISA Kit, zeta-chain (TCR) associated protein kinase 70kDa ELISA Kit, zeta chain of T cell receptor associated protein kinase 70kDa L homeolog ELISA Kit, zeta chain of T cell receptor associated protein kinase 70kDa ELISA Kit, tyrosine-protein kinase ZAP-70 ELISA Kit, ZAP70 ELISA Kit, Smp_149470 ELISA Kit, Zap70 ELISA Kit, zap70.L ELISA Kit, zap70 ELISA Kit, LOC707555 ELISA Kit, LOC100342021 ELISA Kit, LOC100390922 ELISA Kit, LOC100542877 ELISA Kit
    基因ID
    7535
    UniProt
    P43403
    途径
    TCR Signaling, Ubiquitin Proteasome Pathway
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