ZAP70 ELISA 试剂盒
ZAP70
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
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- 抗原 See all ZAP70 ELISA试剂盒
- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human ZAP70 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes .
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) ELISA Kit
ZAP70 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all ZAP70 ELISA试剂盒
- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
- 别名
- ZAP70 (ZAP70 产品)
- 别名
- SRK ELISA Kit, STCD ELISA Kit, STD ELISA Kit, TZK ELISA Kit, ZAP-70 ELISA Kit, 70kDa ELISA Kit, AI327364 ELISA Kit, Srk ELISA Kit, mrtle ELISA Kit, mur ELISA Kit, zap70 ELISA Kit, MGC80670 ELISA Kit, MGC88962 ELISA Kit, ZAP70 ELISA Kit, zgc:110383 ELISA Kit, zeta chain of T-cell receptor associated protein kinase 70 ELISA Kit, putative tyrosine-protein kinase zap-70 ELISA Kit, zeta-chain (TCR) associated protein kinase ELISA Kit, zeta chain of T cell receptor associated protein kinase 70 ELISA Kit, zeta-chain (TCR) associated protein kinase 70kDa ELISA Kit, zeta chain of T cell receptor associated protein kinase 70kDa L homeolog ELISA Kit, zeta chain of T cell receptor associated protein kinase 70kDa ELISA Kit, tyrosine-protein kinase ZAP-70 ELISA Kit, ZAP70 ELISA Kit, Smp_149470 ELISA Kit, Zap70 ELISA Kit, zap70.L ELISA Kit, zap70 ELISA Kit, LOC707555 ELISA Kit, LOC100342021 ELISA Kit, LOC100390922 ELISA Kit, LOC100542877 ELISA Kit
- 基因ID
- 7535
- UniProt
- P43403
- 途径
- TCR Signaling, Ubiquitin Proteasome Pathway
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