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WNT4 ELISA 试剂盒
WNT4
适用: 人
Colorimetric
Sandwich ELISA
1.22-300 ng/mL
Cell Culture Supernatant, Plasma, Serum
WNT4ELISA Kit详情
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WNT4
(Wingless-Type MMTV Integration Site Family, Member 4 (WNT4))
适用
All reactivities for WNT4 ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
1.22-300 ng/mL
最低检测浓度
1.22 ng/mL
应用范围
ELISA
原理
Human Wnt-4 ELISA Kit for serum, plasma, and cell culture supernatant samples.
样品类型
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
特异性
This ELISA antibody pair detects human Wnt-4. Other species not determined.
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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应用备注
Recommended Dilution for serum and plasma samples2 fold
样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
WNT4目标详情
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WNT4
(Wingless-Type MMTV Integration Site Family, Member 4 (WNT4))
别名
Wnt-4 (WNT4 产品 )
别名
CG4698 ELISA Kit, DWnt-4 ELISA Kit, DWnt4 ELISA Kit, Dm DWnt4 ELISA Kit, Dmel\\CG4698 ELISA Kit, Dwnt4 ELISA Kit, Wnt ELISA Kit, Wnt-4 ELISA Kit, anon-EST:Liang-2.4 ELISA Kit, clone 2.4 ELISA Kit, wnt-4 ELISA Kit, wnt4 ELISA Kit, SERKAL ELISA Kit, WNT-4 ELISA Kit, Xwnt4 ELISA Kit, xwnt-4 ELISA Kit, zgc:136737 ELISA Kit, Wnt family member 4 ELISA Kit, Wnt oncogene analog 4 ELISA Kit, wingless-type MMTV integration site family, member 4 ELISA Kit, wingless-type MMTV integration site family member 4 S homeolog ELISA Kit, wingless-type MMTV integration site family, member 4a ELISA Kit, WNT4 ELISA Kit, Wnt4 ELISA Kit, wnt4.S ELISA Kit, wnt4a ELISA Kit
基因ID
54361
UniProt
P56705
途径
WNT signaling , Regulation of Hormone Metabolic Process , Regulation of Hormone Biosynthetic Process , Cell-Cell Junction Organization , Tube Formation
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