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UCHL1 ELISA 试剂盒
UCHL1
适用: 人
Colorimetric
Sandwich ELISA
0.82-200 ng/mL
Cell Culture Supernatant, Plasma, Serum
UCHL1ELISA Kit详情
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UCHL1
(Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
适用
All reactivities for UCHL1 ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.82-200 ng/mL
最低检测浓度
0.82 ng/mL
应用范围
ELISA
原理
Human UCH-L1/PGP9.5 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
样品类型
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
特异性
This ELISA antibody pair recognizes Human UCH-L1.
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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应用备注
Recommended Dilution for serum and plasma samples2 fold
样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
UCHL1目标详情
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UCHL1
(Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
别名
UCH-L1 (UCHL1 产品 )
别名
PARK5 ELISA Kit, PGP 9.5 ELISA Kit, PGP9.5 ELISA Kit, PGP95 ELISA Kit, Uch-L1 ELISA Kit, UCH-L1 ELISA Kit, UCHL1 ELISA Kit, cb358 ELISA Kit, wu:fc55h08 ELISA Kit, park5 ELISA Kit, pgp9.5 ELISA Kit, uch-l1 ELISA Kit, MGC132191 ELISA Kit, uchl1 ELISA Kit, AW822034 ELISA Kit, C88048 ELISA Kit, R75593 ELISA Kit, UCHL-1 ELISA Kit, gad ELISA Kit, ubiquitin C-terminal hydrolase L1 ELISA Kit, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) ELISA Kit, ubiquitin C-terminal hydrolase L1 L homeolog ELISA Kit, ubiquitin carboxyl-terminal esterase L1 ELISA Kit, ubiquitin carboxy-terminal hydrolase L1 ELISA Kit, UCHL1 ELISA Kit, uchl1 ELISA Kit, uchl1.L ELISA Kit, LOC100304750 ELISA Kit, Uchl1 ELISA Kit
基因ID
7345
UniProt
P09936
途径
Feeding Behaviour
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