THBS4 ELISA 试剂盒
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- 抗原 See all THBS4 ELISA试剂盒
- THBS4 (Thrombospondin 4 (THBS4))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.1-25 ng/mL
- 最低检测浓度
- 0.1 ng/mL
- 应用范围
- ELISA
- 原理
- Human Thrombospondin-4 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human Thrombospondin-4. Other species not yet determined.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Thrombospondin 4 (THBS4) ELISA Kit
THBS4 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma
Thrombospondin 4 (THBS4) ELISA KitTHBS4 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Tissue Homogenate
Thrombospondin 4 (THBS4) ELISA KitTHBS4 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Thrombospondin 4 (THBS4) ELISA KitTHBS4 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples100 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all THBS4 ELISA试剂盒
- THBS4 (Thrombospondin 4 (THBS4))
- 别名
- Thrombospondin-4 (THBS4 产品)
- 别名
- TSP4 ELISA Kit, tsp4 ELISA Kit, thbs4 ELISA Kit, zgc:111910 ELISA Kit, MGC79977 ELISA Kit, TSP-4 ELISA Kit, zgc:175276 ELISA Kit, thrombospondin 4 ELISA Kit, thrombospondin 4b ELISA Kit, thrombospondin 4 S homeolog ELISA Kit, thrombospondin-4 ELISA Kit, thrombospondin 4a ELISA Kit, THBS4 ELISA Kit, Thbs4 ELISA Kit, thbs4b ELISA Kit, thbs4.S ELISA Kit, thbs4 ELISA Kit, thbs4a ELISA Kit
- 基因ID
- 7060
- UniProt
- P35443
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