TSKU ELISA 试剂盒
TSKU
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
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- 抗原 See all TSKU ELISA试剂盒
- TSKU (Tsukushin (TSKU))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human Tsukushi/TSK ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes .
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all TSKU ELISA试剂盒
- TSKU (Tsukushin (TSKU))
- 别名
- Tsukushi (TSKU 产品)
- 别名
- LRRC54 ELISA Kit, tsk ELISA Kit, tsku ELISA Kit, X-TSK ELISA Kit, e2ig4 ELISA Kit, lrrc54 ELISA Kit, tsukushi ELISA Kit, x-tsk-b1 ELISA Kit, tsukushin ELISA Kit, x-tsk-b2 ELISA Kit, c-tsk ELISA Kit, 9530051K01Rik ELISA Kit, E2ig4 ELISA Kit, Lrrc54 ELISA Kit, Tsk ELISA Kit, C-TSK ELISA Kit, C-TSKB ELISA Kit, EIIH ELISA Kit, fd51d02 ELISA Kit, wu:fd51d02 ELISA Kit, zgc:56201 ELISA Kit, E2IG4 ELISA Kit, TSK ELISA Kit, Eiih ELISA Kit, tsukushi, small leucine rich proteoglycan ELISA Kit, tsukushi, small leucine rich proteoglycan L homeolog ELISA Kit, tsukushi, small leucine rich proteoglycan S homeolog ELISA Kit, tsukushi small leucine rich proteoglycan homolog (Xenopus laevis) ELISA Kit, TSKU ELISA Kit, tsku.L ELISA Kit, tsku.S ELISA Kit, tsku ELISA Kit, Tsku ELISA Kit
- 基因ID
- 25987
- UniProt
- Q8WUA8
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