ACP5 ELISA 试剂盒
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- 抗原 See all ACP5 ELISA试剂盒
- ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human Tartrate Resistant Acid Phosphatase 5 (ACP5 / PAP) ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Human Tartrate Resistant Acid Phosphatase 5 (ACP5 / PAP)
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA Kit
ACP5 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA KitACP5 适用: 大鼠 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA KitACP5 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA KitACP5 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA KitACP5 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA KitACP5 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA KitSmall Sample ACP5 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA KitSmall Sample ACP5 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all ACP5 ELISA试剂盒
- ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))
- 别名
- TRACP (ACP5 产品)
- 别名
- SPENCDI ELISA Kit, TRAP ELISA Kit, acp5 ELISA Kit, sb:cb576 ELISA Kit, zgc:63825 ELISA Kit, wu:fb19f01 ELISA Kit, wu:fb30b03 ELISA Kit, wu:fi14e01 ELISA Kit, wu:fj66f03 ELISA Kit, MGC78938 ELISA Kit, MGC89674 ELISA Kit, TR-AP ELISA Kit, zgc:92339 ELISA Kit, TRACP ELISA Kit, TTRRAP ELISA Kit, Trap ELISA Kit, UF ELISA Kit, acid phosphatase 5, tartrate resistant ELISA Kit, acid phosphatase 5a, tartrate resistant ELISA Kit, acid phosphatase 5, tartrate resistant S homeolog ELISA Kit, tartrate resistant acid phosphatase ELISA Kit, Tartrate-resistant acid phosphatase type 5 ELISA Kit, tartrate-resistant acid phosphatase type 5 ELISA Kit, acid phosphatase 5b, tartrate resistant ELISA Kit, ACP5 ELISA Kit, acp5a ELISA Kit, acp5.S ELISA Kit, acp5 ELISA Kit, NAEGRDRAFT_81291 ELISA Kit, ppa5 ELISA Kit, LOC100282899 ELISA Kit, acp5b ELISA Kit, Acp5 ELISA Kit
- 基因ID
- 54
- UniProt
- P13686
- 途径
- Transition Metal Ion Homeostasis
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