Thimet Oligopeptidase 1 ELISA 试剂盒
THOP1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
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- 抗原 See all Thimet Oligopeptidase 1 (THOP1) ELISA试剂盒
- Thimet Oligopeptidase 1 (THOP1)
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human Thimet Oligopeptidase (THOP1) ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Thimet Oligopeptidase 1 (THOP1) ELISA Kit
THOP1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Thimet Oligopeptidase 1 (THOP1) ELISA KitTHOP1 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all Thimet Oligopeptidase 1 (THOP1) ELISA试剂盒
- Thimet Oligopeptidase 1 (THOP1)
- 别名
- Thimet Oligopeptidase (THOP1 产品)
- 别名
- EP24.15 ELISA Kit, MEPD_HUMAN ELISA Kit, MP78 ELISA Kit, TOP ELISA Kit, AI131655 ELISA Kit, AI327041 ELISA Kit, THOP1 ELISA Kit, thop1 ELISA Kit, MGC147297 ELISA Kit, LOC397806 ELISA Kit, mepd ELISA Kit, thimet oligopeptidase 1 ELISA Kit, thimet oligopeptidase 1 L homeolog ELISA Kit, thimet oligopeptidase ELISA Kit, peptidase ELISA Kit, Thimet oligopeptidase ELISA Kit, THOP1 ELISA Kit, Thop1 ELISA Kit, thop1 ELISA Kit, thop1.L ELISA Kit, Tc00.1047053506411.10 ELISA Kit, Tc00.1047053511237.10 ELISA Kit, Noca_4613 ELISA Kit, ANI_1_1400064 ELISA Kit, MBOO_RS10705 ELISA Kit, AOR_1_880174 ELISA Kit, AnaeK_0425 ELISA Kit, MPAL_RS03920 ELISA Kit, Hoch_5061 ELISA Kit, mepd ELISA Kit
- 基因ID
- 7064
- UniProt
- P52888
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