R-Spondin 3 ELISA 试剂盒
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- 抗原 See all R-Spondin 3 (RSPO3) ELISA试剂盒
- R-Spondin 3 (RSPO3)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human R-Spondin 3 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes .
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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R-Spondin 3 (RSPO3) ELISA Kit
RSPO3 适用: 小鼠 Colorimetric Sandwich ELISA 4.7 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate
R-Spondin 3 (RSPO3) ELISA KitRSPO3 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
R-Spondin 3 (RSPO3) ELISA KitRSPO3 适用: 人 AA 22-272 Colorimetric Sandwich ELISA
R-Spondin 3 (RSPO3) ELISA KitRSPO3 适用: 小鼠 AA 22-277 Colorimetric Sandwich ELISA
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all R-Spondin 3 (RSPO3) ELISA试剂盒
- R-Spondin 3 (RSPO3)
- 别名
- R-Spondin 3 (RSPO3 产品)
- 别名
- pwtsr ELISA Kit, thsd2 ELISA Kit, cristin1 ELISA Kit, CRISTIN1 ELISA Kit, PWTSR ELISA Kit, THSD2 ELISA Kit, RGD1563246 ELISA Kit, 2810459H04Rik ELISA Kit, AW742308 ELISA Kit, Cristin1 ELISA Kit, Thsd2 ELISA Kit, R-spondin 3 ELISA Kit, rspo3 ELISA Kit, RSPO3 ELISA Kit, Rspo3 ELISA Kit
- 基因ID
- 84870
- UniProt
- Q9BXY4
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