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Ret Proto-Oncogene ELISA 试剂盒

RET 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
产品编号 ABIN4884350
发货至: 中国
  • 抗原 See all Ret Proto-Oncogene (RET) ELISA试剂盒
    Ret Proto-Oncogene (RET)
    适用
    • 5
    • 3
    • 2
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Human Ret ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes human Ret. Other species not yet determined.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all Ret Proto-Oncogene (RET) ELISA试剂盒
    Ret Proto-Oncogene (RET)
    别名
    Ret (RET 产品)
    别名
    c-ret ELISA Kit, cret ELISA Kit, etID315074.13 ELISA Kit, ret1 ELISA Kit, wu:fd13h01 ELISA Kit, X-ret ELISA Kit, ret-A ELISA Kit, xret ELISA Kit, RET ELISA Kit, MTC1 ELISA Kit, CDHF12 ELISA Kit, CDHR16 ELISA Kit, HSCR1 ELISA Kit, MEN2A ELISA Kit, MEN2B ELISA Kit, PTC ELISA Kit, RET-ELE1 ELISA Kit, RET51 ELISA Kit, RET9 ELISA Kit, c-Ret ELISA Kit, ret proto-oncogene receptor tyrosine kinase ELISA Kit, ret proto-oncogene S homeolog ELISA Kit, ret proto-oncogene ELISA Kit, ret ELISA Kit, ret.S ELISA Kit, RET ELISA Kit, Ret ELISA Kit
    基因ID
    5979
    UniProt
    P07949
    途径
    RTK signaling, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation
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