PTH1R ELISA 试剂盒
PTH1R
适用: 人
Colorimetric
Sandwich ELISA
8.19-2.000 pg/mL
Cell Culture Supernatant, Plasma, Serum
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- 抗原 See all PTH1R ELISA试剂盒
- PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 8.19-2.000 pg/mL
- 最低检测浓度
- 8.19 pg/mL
- 应用范围
- ELISA
- 原理
- Human PTH1R ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human PTH1R.
- 灵敏度
- 8.2 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Parathyroid Hormone 1 Receptor (PTH1R) ELISA Kit
PTH1R 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Parathyroid Hormone 1 Receptor (PTH1R) ELISA KitPTH1R 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all PTH1R ELISA试剂盒
- PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))
- 别名
- PTH1R (PTH1R 产品)
- 别名
- PFE ELISA Kit, PTHR ELISA Kit, PTHR1 ELISA Kit, PPR ELISA Kit, Pthr ELISA Kit, Pthr1 ELISA Kit, pthr1 ELISA Kit, zPTH1R ELISA Kit, PTHrel ELISA Kit, PTH1 ELISA Kit, PTH ELISA Kit, parathyroid hormone 1 receptor ELISA Kit, parathyroid hormone 1 receptor a ELISA Kit, PTH1R ELISA Kit, Pth1r ELISA Kit, pth1ra ELISA Kit
- 基因ID
- 5745
- UniProt
- Q03431
- 途径
- Regulation of Carbohydrate Metabolic Process
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