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PAM ELISA 试剂盒

PAM 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
产品编号 ABIN4884076
发货至: 中国
  • 抗原 See all PAM ELISA试剂盒
    PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))
    适用
    • 3
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Human PAM ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes .
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all PAM ELISA试剂盒
    PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))
    别名
    PAM (PAM 产品)
    别名
    PAL ELISA Kit, PHM ELISA Kit, pal ELISA Kit, phm ELISA Kit, pamb ELISA Kit, CG3832 ELISA Kit, Dmel\\CG3832 ELISA Kit, dPHM ELISA Kit, AE-I ELISA Kit, PAL-A ELISA Kit, PHM-A ELISA Kit, pama ELISA Kit, si:dkeyp-35f12.2 ELISA Kit, peptidylglycine alpha-amidating monooxygenase ELISA Kit, peptidylglycine alpha-amidating monooxygenase S homeolog ELISA Kit, Peptidylglycine-alpha-hydroxylating monooxygenase ELISA Kit, peptidylglycine alpha-amidating monooxygenase L homeolog ELISA Kit, PAM ELISA Kit, Pam ELISA Kit, pam.S ELISA Kit, CHLREDRAFT_150435 ELISA Kit, sce7070 ELISA Kit, pam ELISA Kit, Phm ELISA Kit, pam.L ELISA Kit
    基因ID
    5066
    UniProt
    P19021
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