RPS6KB1 ELISA 试剂盒
RPS6KB1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
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- 抗原 See all RPS6KB1 ELISA试剂盒
- RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human p70 S6 Kinase ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes .
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) ELISA Kit
RPS6KB1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all RPS6KB1 ELISA试剂盒
- RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))
- 别名
- p70 S6 Kinase (RPS6KB1 产品)
- 别名
- PS6K ELISA Kit, S6K ELISA Kit, S6K-beta-1 ELISA Kit, S6K1 ELISA Kit, STK14A ELISA Kit, p70 S6KA ELISA Kit, p70(S6K)-alpha ELISA Kit, p70-S6K ELISA Kit, p70-alpha ELISA Kit, 10539 ELISA Kit, 7084 ELISA Kit, CG10539 ELISA Kit, DS6K ELISA Kit, Dmel\\CG10539 ELISA Kit, Dp70S6k ELISA Kit, Dp70[s6k] ELISA Kit, Dp70s6k ELISA Kit, S6k ELISA Kit, dS6K ELISA Kit, dS6k ELISA Kit, dp70[S6k] ELISA Kit, dp70s6k ELISA Kit, dps6k ELISA Kit, ds6k ELISA Kit, fs(3)07084 ELISA Kit, l(3)07084 ELISA Kit, p70 S6K ELISA Kit, p70/S6K ELISA Kit, p70S6K ELISA Kit, p70[S6 kinase] ELISA Kit, p70[S6K] ELISA Kit, p70[S6k] ELISA Kit, p70[S6kinase] ELISA Kit, p70s6K ELISA Kit, s6k ELISA Kit, s6k11 ELISA Kit, 2610318I15Rik ELISA Kit, 4732464A07Rik ELISA Kit, 70kDa ELISA Kit, AA959758 ELISA Kit, AI256796 ELISA Kit, AI314060 ELISA Kit, p70/85s6k ELISA Kit, p70s6k ELISA Kit, p70s6k-A ELISA Kit, p70-s6k ELISA Kit, ps6k ELISA Kit, rps6kb1 ELISA Kit, rps6kb1-A ELISA Kit, s6K1 ELISA Kit, stk14a ELISA Kit, fc51h01 ELISA Kit, wu:fc51h01 ELISA Kit, zgc:55713 ELISA Kit, ribosomal protein S6 kinase B1 ELISA Kit, Ribosomal protein S6 kinase ELISA Kit, ribosomal protein S6 kinase, polypeptide 1 ELISA Kit, ribosomal protein S6 kinase B1 L homeolog ELISA Kit, ribosomal protein S6 kinase B1 S homeolog ELISA Kit, ribosomal protein S6 kinase b, polypeptide 1b ELISA Kit, RPS6KB1 ELISA Kit, S6k ELISA Kit, Rps6kb1 ELISA Kit, rps6kb1.L ELISA Kit, rps6kb1.S ELISA Kit, rps6kb1b ELISA Kit
- 基因ID
- 6198
- UniProt
- P23443
- 途径
- PI3K-Akt Signaling, RTK signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, Warburg Effect
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