Norrie Disease (Pseudoglioma) ELISA 试剂盒
NDP
适用: 人
Colorimetric
Sandwich ELISA
0.41-100 ng/mL
Cell Culture Supernatant, Plasma, Serum
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- 抗原 See all Norrie Disease (Pseudoglioma) (NDP) ELISA试剂盒
- Norrie Disease (Pseudoglioma) (NDP)
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.41-100 ng/mL
- 最低检测浓度
- 0.41 ng/mL
- 应用范围
- ELISA
- 原理
- Human Norrin ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human Norrin.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Norrie Disease (Pseudoglioma) (NDP) ELISA Kit
NDP 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all Norrie Disease (Pseudoglioma) (NDP) ELISA试剂盒
- Norrie Disease (Pseudoglioma) (NDP)
- 别名
- Norrin (NDP 产品)
- 别名
- EVR2 ELISA Kit, FEVR ELISA Kit, ND ELISA Kit, evr2 ELISA Kit, fevr ELISA Kit, xnorrin ELISA Kit, Ndph ELISA Kit, RGD1563968 ELISA Kit, NDP, norrin cystine knot growth factor ELISA Kit, Norrie disease (pseudoglioma) L homeolog ELISA Kit, Norrie disease (pseudoglioma) (human) ELISA Kit, NDP ELISA Kit, ndp.L ELISA Kit, Ndp ELISA Kit
- 基因ID
- 4693
- UniProt
- Q00604
- 途径
- Sensory Perception of Sound
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