KIRREL3 ELISA 试剂盒
KIRREL3
适用: 人
Colorimetric
Sandwich ELISA
0.21-50 ng/mL
Cell Culture Supernatant, Plasma, Serum
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- 抗原 See all KIRREL3 ELISA试剂盒
- KIRREL3 (Kin of IRRE Like 3 (KIRREL3))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.21-50 ng/mL
- 最低检测浓度
- 0.21 ng/mL
- 应用范围
- ELISA
- 原理
- Human KIRREL3 (NEPH2) ELISA Kit for serum, plasma, and cell culture supernatant samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human 4-1BB Ligand, Activin RIIB, Aminopeptidase P2, BAMBI, BOC, Brevican, Carbonic Anhydrase XII, Carboxypeptidase A2, CD300c (LMIR2), CD320 (TCblR, 8D6A), CDNF, CDO, CHST1, CHST4, CILP-1, CNTF R alpha, CRIM1, CRTAC1, CXADR, Dopa Decarboxylase, DPPII, DSPG3, EMR2, FCAR (CD89), FCRL1 (FcRH1), FCRL2 (FcRH2), Gas6, GPR56, GPVI, Hepsin, ILT2 (CD85j), Jagged 2, KLF4, LAIR1, LAMP, LAMP1 (CD107a),MDGA1, MIS RII, Neurexin
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product KIRREL3 IQ-ELISA Kit
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all KIRREL3 ELISA试剂盒
- KIRREL3 (Kin of IRRE Like 3 (KIRREL3))
- 别名
- KIRREL3///NEPH2 (KIRREL3 产品)
- 别名
- KIRRE ELISA Kit, MRD4 ELISA Kit, NEPH2 ELISA Kit, PRO4502 ELISA Kit, 1500010O20Rik ELISA Kit, 2900036G11Rik ELISA Kit, SST4 ELISA Kit, mKIAA1867 ELISA Kit, kirre like nephrin family adhesion molecule 3 ELISA Kit, kin of IRRE like 3 (Drosophila) ELISA Kit, KIRREL3 ELISA Kit, Kirrel3 ELISA Kit
- 基因ID
- 84623
- UniProt
- Q8IZU9
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