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ING1 ELISA 试剂盒

ING1 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
产品编号 ABIN4883452
发货至: 中国
  • 抗原 See all ING1 ELISA试剂盒
    ING1 (Inhibitor of Growth Family, Member 1 (ING1))
    适用
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Human ING1 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes .
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product ING1 IQ-ELISA Kit
  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all ING1 ELISA试剂盒
    ING1 (Inhibitor of Growth Family, Member 1 (ING1))
    别名
    ING1 (ING1 产品)
    别名
    p24ING1c ELISA Kit, p33 ELISA Kit, p33ING1 ELISA Kit, p33ING1b ELISA Kit, p47 ELISA Kit, p47ING1a ELISA Kit, p33ING1c ELISA Kit, zgc:136918 ELISA Kit, ING1 ELISA Kit, 2610028J21Rik ELISA Kit, AA407184 ELISA Kit, AI875420 ELISA Kit, mING1h ELISA Kit, p33Ing1 ELISA Kit, p37Ing1b ELISA Kit, xing1 ELISA Kit, inhibitor of growth family member 1 ELISA Kit, inhibitor of growth family, member 1 ELISA Kit, inhibitor of growth family member 1 S homeolog ELISA Kit, ING1 ELISA Kit, Ing1 ELISA Kit, ing1 ELISA Kit, ing1.S ELISA Kit
    基因ID
    3621
    UniProt
    Q9UK53
    途径
    Protein targeting to Nucleus, Autophagy
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