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IFNGR1 ELISA 试剂盒
IFNGR1
适用: 人
Colorimetric
Sandwich ELISA
24-6000 pg/mL
Cell Culture Supernatant, Plasma, Serum
IFNGR1ELISA Kit详情
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IFNGR1
(Interferon gamma Receptor 1 (IFNGR1))
适用
All reactivities for IFNGR1 ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
24-6000 pg/mL
最低检测浓度
24 pg/mL
应用范围
ELISA
原理
Human IFN-gamma R1 ELISA Kit for serum, plasma, and cell culture supernatant samples.
样品类型
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
特异性
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ALK-1, B7-H2, BLAME, BMP-8, CD28, Common beta Chain (CD131), Contactin-1, Desmoglein-1, Desmoglein-3, EDAR, EphA1, EphB6, Ephrin-B3, Epiregulin, FGF-12, FGF-17, FOLR2, Galectin-8, Glypican 1, Glypican 5, GHR, IL-22 R alpha 1, IL-22BP, IL-23 R, IL-31 RA, IL-7R alpha, Integrin alpha 5, MDM2, Nectin-1, NKp30, Nogo Receptor, Notch-3, OSM R beta, Prolactin R, RELT, Ryk, Semaphorin 6D, Semaphorin 7A, Siglec-11.
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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应用备注
Recommended Dilution for serum and plasma samples2 fold
样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
IFNGR1目标详情
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抗原
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IFNGR1
(Interferon gamma Receptor 1 (IFNGR1))
别名
IFN-gamma R1 (IFNGR1 产品 )
别名
CD119 ELISA Kit, IFNGR ELISA Kit, IFN-gammaR ELISA Kit, Ifgr ELISA Kit, Ifngr ELISA Kit, Nktar ELISA Kit, IFNGR1 ELISA Kit, MGC129098 ELISA Kit, ifngr1 ELISA Kit, MGC194858 ELISA Kit, MGC194873 ELISA Kit, zgc:194858 ELISA Kit, cd119 ELISA Kit, ifngr ELISA Kit, DKFZp469K1232 ELISA Kit, interferon gamma receptor 1 ELISA Kit, cytokine receptor family member B17 ELISA Kit, IFNGR1 ELISA Kit, Ifngr1 ELISA Kit, crfb17 ELISA Kit, ifngr1 ELISA Kit
基因ID
3459
UniProt
P15260
途径
Interferon-gamma Pathway
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