HMGB3 ELISA 试剂盒
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- 抗原 See all HMGB3 ELISA试剂盒
- HMGB3 (High Mobility Group Box 3 (HMGB3))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human HMGB3 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes .
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling HMGB3 ELISA Kit
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High Mobility Group Box 3 (HMGB3) ELISA Kit
HMGB3 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
High Mobility Group Box 3 (HMGB3) ELISA KitHMGB3 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum
High Mobility Group Box 3 (HMGB3) ELISA KitHMGB3 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all HMGB3 ELISA试剂盒
- HMGB3 (High Mobility Group Box 3 (HMGB3))
- 别名
- HMGB3 (HMGB3 产品)
- 别名
- HMG-2a ELISA Kit, HMG-4 ELISA Kit, HMG2A ELISA Kit, HMG4 ELISA Kit, Hmg2a ELISA Kit, Hmg4 ELISA Kit, RGD1564407 ELISA Kit, hmgb3 ELISA Kit, MGC54022 ELISA Kit, Xhmgb3 ELISA Kit, MGC88931 ELISA Kit, fa19b06 ELISA Kit, fj43d02 ELISA Kit, wu:fa19b06 ELISA Kit, wu:fj43d02 ELISA Kit, zgc:112073 ELISA Kit, HMGB3 ELISA Kit, NFD03 ELISA Kit, NFD3 ELISA Kit, NUCLEOSOME/CHROMATIN ASSEMBLY FACTOR ELISA Kit, high mobility group B3 ELISA Kit, HMG-1 ELISA Kit, HMG2a ELISA Kit, HMGB1 ELISA Kit, high mobility group box 3 ELISA Kit, high mobility group box 3b ELISA Kit, high mobility group box 3 S homeolog ELISA Kit, high mobility group protein ELISA Kit, high mobility group B3 ELISA Kit, High mobility group protein B3 ELISA Kit, HMGB3 ELISA Kit, Hmgb3 ELISA Kit, hmgb3 ELISA Kit, hmgb3b ELISA Kit, hmgb3.S ELISA Kit
- 基因ID
- 3149
- UniProt
- O15347
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