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Hexosaminidase A ELISA 试剂盒

HEXA 适用: 人 Colorimetric Sandwich ELISA 0.082-20 ng/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN4883183
发货至: 中国
  • 抗原 See all Hexosaminidase A (HEXA) ELISA试剂盒
    Hexosaminidase A (HEXA)
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.082-20 ng/mL
    最低检测浓度
    0.082 ng/mL
    应用范围
    ELISA
    原理
    Human HEXA ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human HEXA.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 应用备注
    Recommended Dilution for serum and plasma samples10 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all Hexosaminidase A (HEXA) ELISA试剂盒
    Hexosaminidase A (HEXA)
    别名
    HEXA (HEXA 产品)
    别名
    TSD ELISA Kit, fc04h11 ELISA Kit, wu:fc04h11 ELISA Kit, wu:fv09f06 ELISA Kit, si:dkey-35i22.2 ELISA Kit, HEXA ELISA Kit, Hex-1 ELISA Kit, zgc:112084 ELISA Kit, hexosaminidase subunit alpha ELISA Kit, hexosaminidase B (beta polypeptide) ELISA Kit, CUGBP Elav-like family member 6 ELISA Kit, hexosaminidase A ELISA Kit, hexosaminidase A (alpha polypeptide) ELISA Kit, beta-hexosaminidase subunit alpha-like ELISA Kit, HEXA ELISA Kit, hexb ELISA Kit, CELF6 ELISA Kit, Hexa ELISA Kit, hexa ELISA Kit, LOC100228061 ELISA Kit
    基因ID
    3073
    UniProt
    P06865
    途径
    Sensory Perception of Sound, Glycosaminoglycan Metabolic Process
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