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HAGH ELISA 试剂盒

HAGH 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
产品编号 ABIN4883097
发货至: 中国
  • 抗原 See all HAGH ELISA试剂盒
    HAGH (Hydroxyacylglutathione Hydrolase (HAGH))
    适用
    • 3
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    • 2
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Human Glyoxalase II ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes .
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all HAGH ELISA试剂盒
    HAGH (Hydroxyacylglutathione Hydrolase (HAGH))
    别名
    Glyoxalase II (HAGH 产品)
    别名
    glo-2 ELISA Kit, glo2 ELISA Kit, glx2 ELISA Kit, glxii ELISA Kit, hagh1 ELISA Kit, DDBDRAFT_0186675 ELISA Kit, DDBDRAFT_0230988 ELISA Kit, DDB_0186675 ELISA Kit, DDB_0230988 ELISA Kit, DDBDRAFT_0183924 ELISA Kit, DDBDRAFT_0230991 ELISA Kit, DDB_0183924 ELISA Kit, DDB_0230991 ELISA Kit, BC019817 ELISA Kit, Glo-2 ELISA Kit, Glo2 ELISA Kit, Rsp29 ELISA Kit, RSP29 ELISA Kit, fa66a03 ELISA Kit, wu:fa66a03 ELISA Kit, zgc:73161 ELISA Kit, GLO2 ELISA Kit, GLX2 ELISA Kit, GLXII ELISA Kit, HAGH1 ELISA Kit, hydroxyacylglutathione hydrolase L homeolog ELISA Kit, hydroxyacylglutathione hydrolase ELISA Kit, beta-lactamase domain-containing protein ELISA Kit, hydroxyacyl glutathione hydrolase ELISA Kit, hagh.L ELISA Kit, CND02510 ELISA Kit, gloB1 ELISA Kit, gloB2 ELISA Kit, Fbal_1466 ELISA Kit, Hagh ELISA Kit, hagh ELISA Kit, HAGH ELISA Kit
    基因ID
    3029
    UniProt
    Q16775
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