GLO1 ELISA 试剂盒
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- 抗原 See all GLO1 ELISA试剂盒
- GLO1 (Glyoxalase I (GLO1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human Glyoxalase I ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes .
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Glyoxalase I (GLO1) ELISA Kit
GLO1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Glyoxalase I (GLO1) ELISA KitGLO1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Glyoxalase I (GLO1) ELISA KitGLO1 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Glyoxalase I (GLO1) ELISA KitGLO1 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Glyoxalase I (GLO1) ELISA KitGLO1 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all GLO1 ELISA试剂盒
- GLO1 (Glyoxalase I (GLO1))
- 别名
- Glyoxalase I (GLO1 产品)
- 别名
- GLOD1 ELISA Kit, GLYI ELISA Kit, 0610009E22Rik ELISA Kit, 1110008E19Rik ELISA Kit, 2510049H23Rik ELISA Kit, AW550643 ELISA Kit, GLY1 ELISA Kit, Glo-1 ELISA Kit, Glo-1r ELISA Kit, Glo-1s ELISA Kit, Glo1-r ELISA Kit, Glo1-s ELISA Kit, Qglo ELISA Kit, cb554 ELISA Kit, zgc:66035 ELISA Kit, wu:fb82g09 ELISA Kit, glod1 ELISA Kit, glyi ELISA Kit, glo1 ELISA Kit, GLO1 ELISA Kit, ATGLX1 ELISA Kit, F12F1.32 ELISA Kit, F12F1_32 ELISA Kit, GLYOXALASE I ELISA Kit, glyoxalase I homolog ELISA Kit, trypanothione-dependent glyoxalase I ELISA Kit, glyoxalase I ELISA Kit, glyoxalase 1 ELISA Kit, glyoxalase 1 L homeolog ELISA Kit, glyoxalase 1 S homeolog ELISA Kit, lactoylglutathione lyase ELISA Kit, glyoxalase/bleomycin resistance protein/dioxygenase superfamily protein ELISA Kit, GLO1 ELISA Kit, Glo1 ELISA Kit, glo1 ELISA Kit, glo1.L ELISA Kit, glo1.S ELISA Kit, GST3 ELISA Kit, GLX1 ELISA Kit, STY1687 ELISA Kit, Bcen_2094 ELISA Kit, gloA ELISA Kit
- 基因ID
- 2739
- UniProt
- Q04760
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