GLI3 ELISA 试剂盒
GLI3
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
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- 抗原 See all GLI3 ELISA试剂盒
- GLI3 (GLI Family Zinc Finger 3 (GLI3))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human GLI-3 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human GLI-3.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product GLI3 IQ-ELISA Kit
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all GLI3 ELISA试剂盒
- GLI3 (GLI Family Zinc Finger 3 (GLI3))
- 别名
- GLI-3 (GLI3 产品)
- 别名
- ACLS ELISA Kit, GCPS ELISA Kit, GLI3-190 ELISA Kit, GLI3FL ELISA Kit, PAP-A ELISA Kit, PAPA ELISA Kit, PAPA1 ELISA Kit, PAPB ELISA Kit, PHS ELISA Kit, PPDIV ELISA Kit, xgli3 ELISA Kit, AI854843 ELISA Kit, AU023367 ELISA Kit, Bph ELISA Kit, Pdn ELISA Kit, Xt ELISA Kit, add ELISA Kit, im:7142603 ELISA Kit, GLI3 ELISA Kit, GLI family zinc finger 3 ELISA Kit, GLI family zinc finger 3 L homeolog ELISA Kit, GLI-Kruppel family member GLI3 ELISA Kit, GLI3 ELISA Kit, Gli3 ELISA Kit, gli3.L ELISA Kit, gli3 ELISA Kit
- 基因ID
- 2737
- UniProt
- P10071
- 途径
- Hedgehog Signaling
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