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GLI3 ELISA 试剂盒

GLI3 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN4883079
发货至: 中国
  • 抗原 See all GLI3 ELISA试剂盒
    GLI3 (GLI Family Zinc Finger 3 (GLI3))
    适用
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human GLI-3 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human GLI-3.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product GLI3 IQ-ELISA Kit
  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all GLI3 ELISA试剂盒
    GLI3 (GLI Family Zinc Finger 3 (GLI3))
    别名
    GLI-3 (GLI3 产品)
    别名
    ACLS ELISA Kit, GCPS ELISA Kit, GLI3-190 ELISA Kit, GLI3FL ELISA Kit, PAP-A ELISA Kit, PAPA ELISA Kit, PAPA1 ELISA Kit, PAPB ELISA Kit, PHS ELISA Kit, PPDIV ELISA Kit, xgli3 ELISA Kit, AI854843 ELISA Kit, AU023367 ELISA Kit, Bph ELISA Kit, Pdn ELISA Kit, Xt ELISA Kit, add ELISA Kit, im:7142603 ELISA Kit, GLI3 ELISA Kit, GLI family zinc finger 3 ELISA Kit, GLI family zinc finger 3 L homeolog ELISA Kit, GLI-Kruppel family member GLI3 ELISA Kit, GLI3 ELISA Kit, Gli3 ELISA Kit, gli3.L ELISA Kit, gli3 ELISA Kit
    基因ID
    2737
    UniProt
    P10071
    途径
    Hedgehog Signaling
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