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EPH Receptor B2 ELISA 试剂盒

EPHB2 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
产品编号 ABIN4882751
发货至: 中国
  • 抗原 See all EPH Receptor B2 (EPHB2) ELISA试剂盒
    EPH Receptor B2 (EPHB2)
    适用
    • 4
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Human EphB2 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human Tenascin X.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all EPH Receptor B2 (EPHB2) ELISA试剂盒
    EPH Receptor B2 (EPHB2)
    别名
    EphB2 (EPHB2 产品)
    别名
    CAPB ELISA Kit, DRT ELISA Kit, EK5 ELISA Kit, EPHT3 ELISA Kit, ERK ELISA Kit, Hek5 ELISA Kit, PCBC ELISA Kit, Tyro5 ELISA Kit, ERK2 ELISA Kit, ERT1 ELISA Kit, MAPK2 ELISA Kit, P42MAPK ELISA Kit, PRKM1 ELISA Kit, PRKM2 ELISA Kit, p38 ELISA Kit, p40 ELISA Kit, p41 ELISA Kit, p41mapk ELISA Kit, RGD1564232 ELISA Kit, Cek5 ELISA Kit, Drt ELISA Kit, ETECK ELISA Kit, Erk ELISA Kit, Nuk ELISA Kit, Prkm5 ELISA Kit, Qek5 ELISA Kit, Sek3 ELISA Kit, cek5 ELISA Kit, ephb2 ELISA Kit, xEphB2 ELISA Kit, si:ch73-160e9.1 ELISA Kit, tpx2 ELISA Kit, wu:fb09e10 ELISA Kit, wu:fc64a03 ELISA Kit, wu:fi18g12 ELISA Kit, zgc:112296 ELISA Kit, EPH receptor B2 ELISA Kit, mitogen-activated protein kinase 1 ELISA Kit, Eph receptor B2 ELISA Kit, EPH receptor B2 L homeolog ELISA Kit, eph receptor B2a ELISA Kit, eph receptor B2b ELISA Kit, EPHB2 ELISA Kit, MAPK1 ELISA Kit, Ephb2 ELISA Kit, ephb2.L ELISA Kit, ephb2a ELISA Kit, ephb2b ELISA Kit
    基因ID
    2048
    UniProt
    P29323
    途径
    RTK signaling, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins
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