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DSCAM ELISA 试剂盒
DSCAM
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
DSCAMELISA Kit详情
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DSCAM
(Down Syndrome Cell Adhesion Molecule (DSCAM))
适用
All reactivities for DSCAM ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Custom Human DSCAM ELISA Kit.
样品类型
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
DSCAM目标详情
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DSCAM
(Down Syndrome Cell Adhesion Molecule (DSCAM))
别名
DSCAM (DSCAM 产品 )
别名
CHD2-42 ELISA Kit, CHD2-52 ELISA Kit, Dscam ELISA Kit, DSCAM ELISA Kit, chd2-42 ELISA Kit, chd2-52 ELISA Kit, dscam ELISA Kit, GB15141 ELISA Kit, 4932410A21Rik ELISA Kit, 43Bc ELISA Kit, CG17800 ELISA Kit, CT39257 ELISA Kit, DScam ELISA Kit, DmDscam ELISA Kit, Dm_2R:13612 ELISA Kit, Dmel\\CG17800 ELISA Kit, Dscam-hv ELISA Kit, Dscam1 ELISA Kit, FBgn0033159 ELISA Kit, Neu1 ELISA Kit, dScam ELISA Kit, l(2)05518 ELISA Kit, l(2)43Bc ELISA Kit, p270 ELISA Kit, DS cell adhesion molecule ELISA Kit, Down syndrome cell adhesion molecule ELISA Kit, Down syndrome cell adhesion molecule a ELISA Kit, Down syndrome cell adhesion molecule 1 ELISA Kit, Down syndrome cell adhesion molecule-like protein Dscam2 ELISA Kit, down syndrome cell adhesion molecule ELISA Kit, Down syndrome cell adhesion molecule L homeolog ELISA Kit, DSCAM ELISA Kit, Dscam ELISA Kit, dscam ELISA Kit, dscama ELISA Kit, Dscam1 ELISA Kit, LOC5573626 ELISA Kit, CpipJ_CPIJ006173 ELISA Kit, dscam.L ELISA Kit
基因ID
1826
UniProt
O60469
途径
Regulation of Cell Size
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