DFFA ELISA 试剂盒
DFFA
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
-
- 抗原 See all DFFA ELISA试剂盒
- DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human DFF45/ICAD ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human S100 A11.
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product DFFA IQ-ELISA Kit
-
-
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all DFFA ELISA试剂盒
- DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
- 别名
- DFF45 (DFFA 产品)
- 别名
- DFF-45 ELISA Kit, DFF1 ELISA Kit, ICAD ELISA Kit, A330085O09Rik ELISA Kit, DFF35 ELISA Kit, Dff45 ELISA Kit, ICAD-L ELISA Kit, ICAD-S ELISA Kit, DFFA ELISA Kit, zgc:92229 ELISA Kit, DNA fragmentation factor subunit alpha ELISA Kit, DNA fragmentation factor, alpha subunit ELISA Kit, DNA fragmentation factor, 45kDa, alpha polypeptide ELISA Kit, DNA fragmentation factor, alpha polypeptide ELISA Kit, DFFA ELISA Kit, Dffa ELISA Kit, dffa ELISA Kit
- 基因ID
- 1676
- UniProt
- O00273
- 途径
- Apoptosis, Caspase Cascade in Apoptosis
-
You are here:
