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DDR1 ELISA 试剂盒
DDR1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
DDR1ELISA Kit详情
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DDR1
(Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))
适用
All reactivities for DDR1 ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Custom Human DDR1 ELISA Kit.
样品类型
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
DDR1目标详情
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DDR1
(Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))
别名
DDR1 (DDR1 产品 )
别名
CG11573 ELISA Kit, CG13984 ELISA Kit, CG13985 ELISA Kit, CG33531 ELISA Kit, CG9488 ELISA Kit, CG9490 ELISA Kit, CT33540 ELISA Kit, DDR ELISA Kit, Dmel\\CG33531 ELISA Kit, Dmel_CG13984 ELISA Kit, 6030432F18 ELISA Kit, AI323681 ELISA Kit, CD167a ELISA Kit, Cak ELISA Kit, Nep ELISA Kit, PTK3A ELISA Kit, CAK ELISA Kit, CD167 ELISA Kit, EDDR1 ELISA Kit, HGK2 ELISA Kit, MCK10 ELISA Kit, NEP ELISA Kit, NTRK4 ELISA Kit, PTK3 ELISA Kit, RTK6 ELISA Kit, TRKE ELISA Kit, Drd1 ELISA Kit, PTK3D ELISA Kit, Discoidin domain receptor ELISA Kit, discoidin domain receptor family, member 1 ELISA Kit, discoidin domain receptor tyrosine kinase 1 ELISA Kit, Ddr ELISA Kit, Ddr1 ELISA Kit, DDR1 ELISA Kit
基因ID
780
UniProt
Q08345
途径
RTK signaling , Smooth Muscle Cell Migration
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