Cyclin E1 ELISA 试剂盒
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- 抗原 See all Cyclin E1 (CCNE1) ELISA试剂盒
- Cyclin E1 (CCNE1)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human Cyclin E1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human Nicastrin.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Cyclin E1 (CCNE1) ELISA Kit
CCNE1 适用: 人 Colorimetric Sandwich ELISA 25 pg/mL - 1600 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cyclin E1 (CCNE1) ELISA KitCCNE1 适用: 大鼠 Colorimetric Sandwich ELISA 31.25-2000 pg/mL Plasma, Serum
Cyclin E1 (CCNE1) ELISA KitCCNE1 适用: 小鼠 Colorimetric Sandwich ELISA 78.125-5000 pg/mL Plasma, Serum
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all Cyclin E1 (CCNE1) ELISA试剂盒
- Cyclin E1 (CCNE1)
- 别名
- Cyclin E1 (CCNE1 产品)
- 别名
- CCNE1 ELISA Kit, ccne ELISA Kit, cyclinE ELISA Kit, CCNE ELISA Kit, AW538188 ELISA Kit, CycE1 ELISA Kit, CYCLE ELISA Kit, Ccne ELISA Kit, cyce2 ELISA Kit, cycE3 ELISA Kit, cyce ELISA Kit, fa18e07 ELISA Kit, wu:fa18e07 ELISA Kit, cyclin E1 ELISA Kit, cyclin E1 L homeolog ELISA Kit, cyclin E1 S homeolog ELISA Kit, CCNE1 ELISA Kit, ccne1 ELISA Kit, Ccne1 ELISA Kit, ccne1.L ELISA Kit, ccne1.S ELISA Kit
- 基因ID
- 898
- UniProt
- P24864
- 途径
- Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases
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