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CD131 ELISA 试剂盒

CSF2RB 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
产品编号 ABIN4882498
发货至: 中国
  • 抗原 See all CD131 (CSF2RB) ELISA试剂盒
    CD131 (CSF2RB) (Colony Stimulating Factor 2 Receptor, Beta (CSF2RB))
    适用
    • 4
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Human Common beta Chain (CSF2RB) ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    The antibody pair provided in this kit recognizes Human Common beta Chain (CSF2RB)
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all CD131 (CSF2RB) ELISA试剂盒
    CD131 (CSF2RB) (Colony Stimulating Factor 2 Receptor, Beta (CSF2RB))
    别名
    Common beta Chain (CSF2RB 产品)
    别名
    Csf2rb1 ELISA Kit, CSF2RB ELISA Kit, cd131 ELISA Kit, cdw131 ELISA Kit, il3rb ELISA Kit, il5rb ELISA Kit, il-3rbc ELISA Kit, CD131 ELISA Kit, CDw131 ELISA Kit, IL3RB ELISA Kit, IL5RB ELISA Kit, SMDP5 ELISA Kit, AI848964 ELISA Kit, AIC2B ELISA Kit, Bc ELISA Kit, Csfgmrb ELISA Kit, Il3r ELISA Kit, Il3rb ELISA Kit, Il3rb1 ELISA Kit, Il5rb ELISA Kit, colony stimulating factor 2 receptor beta common subunit ELISA Kit, colony stimulating factor 2 receptor beta common subunit S homeolog ELISA Kit, cytokine receptor common subunit beta-like ELISA Kit, colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage) ELISA Kit, CSF2RB ELISA Kit, Csf2rb ELISA Kit, csf2rb.S ELISA Kit, CRCBL ELISA Kit, csf2rb ELISA Kit
    基因ID
    1439
    UniProt
    P32927
    途径
    JAK/STAT Signaling
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