CHST2 ELISA 试剂盒
CHST2
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
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- 抗原 See all CHST2 ELISA试剂盒
- CHST2 (Carbohydrate (N-Acetylglucosamine-6-O) Sulfotransferase 2 (CHST2))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human CHST2 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human KIR2DL3.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product CHST2 IQ-ELISA Kit
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all CHST2 ELISA试剂盒
- CHST2 (Carbohydrate (N-Acetylglucosamine-6-O) Sulfotransferase 2 (CHST2))
- 别名
- CHST2 (CHST2 产品)
- 别名
- chst2 ELISA Kit, wu:fj55f04 ELISA Kit, zgc:154070 ELISA Kit, CHST2 ELISA Kit, C6ST ELISA Kit, GST-2 ELISA Kit, GST2 ELISA Kit, Gn6ST-1 ELISA Kit, glcNAc6ST-1 ELISA Kit, AI428561 ELISA Kit, AW121776 ELISA Kit, C130041E03Rik ELISA Kit, Chts2 ELISA Kit, GlcNAc6ST ELISA Kit, Gn6st ELISA Kit, carbohydrate sulfotransferase 2 ELISA Kit, carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2a ELISA Kit, carbohydrate sulfotransferase 4 ELISA Kit, carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2 ELISA Kit, carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2 L homeolog ELISA Kit, CHST2 ELISA Kit, chst2a ELISA Kit, CHST4 ELISA Kit, chst2 ELISA Kit, chst2.L ELISA Kit, Chst2 ELISA Kit
- 基因ID
- 9435
- UniProt
- Q9Y4C5
- 途径
- Glycosaminoglycan Metabolic Process
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