CA5B ELISA 试剂盒
CA5B
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
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- 抗原 See all CA5B ELISA试剂盒
- CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human CA5B ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling CA5B ELISA Kit
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- Discover our top product CA5B ELISA Kit
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Carbonic Anhydrase VB, Mitochondrial (CA5B) ELISA Kit
CA5B 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Carbonic Anhydrase VB, Mitochondrial (CA5B) ELISA KitCA5B 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Saliva, Serum
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all CA5B ELISA试剂盒
- CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))
- 别名
- CA5B (CA5B 产品)
- 别名
- CA-VB ELISA Kit, 7330410H16Rik ELISA Kit, CAVB ELISA Kit, Ca5b ELISA Kit, CarVb ELISA Kit, D730005F19Rik ELISA Kit, zgc:171653 ELISA Kit, CA5B ELISA Kit, ca5b ELISA Kit, carbonic anhydrase 5B ELISA Kit, carbonic anhydrase 5b, mitochondrial ELISA Kit, carbonic anhydrase Va ELISA Kit, carbonic anhydrase 5A ELISA Kit, carbonic anhydrase 5B, mitochondrial ELISA Kit, carbonic anhydrase VB, mitochondrial L homeolog ELISA Kit, CA5B ELISA Kit, Car5b ELISA Kit, Ca5b ELISA Kit, ca5a ELISA Kit, CA5A ELISA Kit, LOC100051095 ELISA Kit, ca5b.L ELISA Kit
- 基因ID
- 11238
- UniProt
- Q9Y2D0
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