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CA1 ELISA 试剂盒
CA1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
CA1ELISA Kit详情
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CA1
(Carbonic Anhydrase I (CA1))
适用
All reactivities for CA1 ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Custom Human CA1 ELISA Kit.
样品类型
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
CA1目标详情
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CA1
(Carbonic Anhydrase I (CA1))
别名
CA1 (CA1 产品 )
别名
CA-I ELISA Kit, CAB ELISA Kit, Car1 ELISA Kit, AW555628 ELISA Kit, Ca1 ELISA Kit, Car-1 ELISA Kit, BG:DS00941.1 ELISA Kit, CAH ELISA Kit, CG7820 ELISA Kit, Dmel\\CG7820 ELISA Kit, Gh7 ELISA Kit, ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3 ELISA Kit, ATBCA1 ELISA Kit, ATSABP3 ELISA Kit, BETA CARBONIC ANHYDRASE 1 ELISA Kit, F4P13.5 ELISA Kit, F4P13_5 ELISA Kit, SABP3 ELISA Kit, SALICYLIC ACID-BINDING PROTEIN 3 ELISA Kit, carbonic anhydrase 1 ELISA Kit, CA1 ELISA Kit, LOC100135826 ELISA Kit, GB15888 ELISA Kit, carbonic anhydrase 1 ELISA Kit, Carbonic anhydrase 1 ELISA Kit, carbonic anhydrase I ELISA Kit, carbonic anhydrase ELISA Kit, CA1 ELISA Kit, Car1 ELISA Kit, CAH1 ELISA Kit, LOC100135826 ELISA Kit, LOC408827 ELISA Kit, Tsp_05114 ELISA Kit, LOC100153915 ELISA Kit, LOC100050192 ELISA Kit, Ca1 ELISA Kit
基因ID
759
UniProt
P00915
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