CD276 ELISA 试剂盒
-
- 抗原 See all CD276 ELISA试剂盒
- CD276
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human B7 Homolog 3 (CD276) ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Human B7 Homolog 3 (CD276)
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling CD276 ELISA Kit
- Top Product
- Discover our top product CD276 ELISA Kit
-
CD276 (CD276) ELISA Kit
CD276 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD276 (CD276) ELISA KitCD276 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Plasma, Serum, Urine
-
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all CD276 ELISA试剂盒
- CD276
- 别名
- B7-H3 (CD276 产品)
- 别名
- sc:d0645 ELISA Kit, zgc:158279 ELISA Kit, 6030411F23Rik ELISA Kit, AU016588 ELISA Kit, B7RP-2 ELISA Kit, B7h3 ELISA Kit, B7H3 ELISA Kit, 4Ig-B7-H3 ELISA Kit, B7-H3 ELISA Kit, CD276 molecule ELISA Kit, CD276 molecule S homeolog ELISA Kit, CD276 antigen ELISA Kit, Cd276 molecule ELISA Kit, CD276 ELISA Kit, cd276.S ELISA Kit, cd276 ELISA Kit, Cd276 ELISA Kit
- 基因ID
- 80381
- UniProt
- Q5ZPR3
- 途径
- Cancer Immune Checkpoints
-
