ICOSLG ELISA 试剂盒
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- 抗原 See all ICOSLG ELISA试剂盒
- ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.41-100 ng/mL
- 最低检测浓度
- 0.41 ng/mL
- 应用范围
- ELISA
- 原理
- Human B7-H2/ICOS Ligand ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human B7-H2.
- 灵敏度
- 0.4 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling ICOSLG ELISA Kit
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- Discover our top product ICOSLG ELISA Kit
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Inducible T-Cell Co-Stimulator Ligand (ICOSLG) ELISA Kit
ICOSLG 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) ELISA KitICOSLG 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Inducible T-Cell Co-Stimulator Ligand (ICOSLG) ELISA KitICOSLG 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all ICOSLG ELISA试剂盒
- ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))
- 别名
- B7-H2 (ICOSLG 产品)
- 别名
- ICOSLG ELISA Kit, AU044799 ELISA Kit, B7-H2 ELISA Kit, B7RP-1 ELISA Kit, B7h ELISA Kit, BG071784 ELISA Kit, GI50 ELISA Kit, GL50 ELISA Kit, GL50-B ELISA Kit, ICOS-L ELISA Kit, Icoslg ELISA Kit, LICOS ELISA Kit, Ly115l ELISA Kit, mKIAA0653 ELISA Kit, B7H2 ELISA Kit, B7RP1 ELISA Kit, CD275 ELISA Kit, ICOSL ELISA Kit, Icosl ELISA Kit, RGD1562791 ELISA Kit, inducible T-cell costimulator ligand ELISA Kit, icos ligand ELISA Kit, inducible T-cell co-stimulator ligand ELISA Kit, ICOSLG ELISA Kit, Icosl ELISA Kit, Icoslg ELISA Kit
- 基因ID
- 10272399623308
- UniProt
- O75144
- 途径
- Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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