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B3GNT2 ELISA 试剂盒
B3GNT2
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
B3GNT2ELISA Kit详情
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抗原
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B3GNT2
(UDP-GlcNAc:BetaGal beta-1,3-N-Acetylglucosaminyltransferase 2 (B3GNT2))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Custom Human B3GNT2 ELISA Kit.
样品类型
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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使用细节
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样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
B3GNT2目标详情
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B3GNT2
(UDP-GlcNAc:BetaGal beta-1,3-N-Acetylglucosaminyltransferase 2 (B3GNT2))
别名
B3GNT2 (B3GNT2 产品 )
别名
B3GN-T2 ELISA Kit, B3GNT ELISA Kit, B3GNT-2 ELISA Kit, B3GNT1 ELISA Kit, BETA3GNT ELISA Kit, BGNT2 ELISA Kit, BGnT-2 ELISA Kit, AA408337 ELISA Kit, AA409404 ELISA Kit, AW260308 ELISA Kit, B3Galt6 ELISA Kit, B3gnt1 ELISA Kit, BGnT-1 ELISA Kit, BGnT1 ELISA Kit, BGnT2 ELISA Kit, Beta3gnt ELISA Kit, MGC81185 ELISA Kit, B3GNT2 ELISA Kit, MGC179257 ELISA Kit, ssp5 ELISA Kit, wu:fa55h04 ELISA Kit, wu:fc39c01 ELISA Kit, zgc:113947 ELISA Kit, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 ELISA Kit, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 L homeolog ELISA Kit, beta-1,4-glucuronyltransferase 1 ELISA Kit, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2b ELISA Kit, B3GNT2 ELISA Kit, B3gnt2 ELISA Kit, b3gnt2.L ELISA Kit, B4GAT1 ELISA Kit, b3gnt2b ELISA Kit, b3gnt2 ELISA Kit
基因ID
10678
UniProt
Q9NY97
途径
Glycosaminoglycan Metabolic Process
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