ARNT ELISA 试剂盒
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- 抗原 See all ARNT ELISA试剂盒
- ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human ARNT/HIF-1 beta ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes .
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) ELISA Kit
ARNT 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) ELISA KitARNT 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) ELISA KitARNT 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all ARNT ELISA试剂盒
- ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))
- 别名
- ARNT (ARNT 产品)
- 别名
- HIF-1-beta ELISA Kit, HIF-1beta ELISA Kit, HIF1-beta ELISA Kit, HIF1B ELISA Kit, HIF1BETA ELISA Kit, TANGO ELISA Kit, bHLHe2 ELISA Kit, D3Ertd557e ELISA Kit, Drnt ELISA Kit, ESTM42 ELISA Kit, Hif1b ELISA Kit, W08714 ELISA Kit, mKIAA4051 ELISA Kit, Arnt1 ELISA Kit, ARNT ELISA Kit, zgc:136664 ELISA Kit, arnt1 ELISA Kit, hif1b ELISA Kit, tango ELISA Kit, xArnt ELISA Kit, hif1beta ELISA Kit, hif-1beta ELISA Kit, arnt.2 ELISA Kit, MGC68805 ELISA Kit, aryl hydrocarbon receptor nuclear translocator ELISA Kit, aryl hydrocarbon receptor nuclear translocator S homeolog ELISA Kit, ARNT ELISA Kit, Arnt ELISA Kit, arnt ELISA Kit, arnt.S ELISA Kit
- 基因ID
- 405
- UniProt
- P27540
- 途径
- Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect
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