Annexin V ELISA 试剂盒
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- 抗原 See all Annexin V (ANXA5) ELISA试剂盒
- Annexin V (ANXA5) (Annexin A5 (ANXA5))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human Annexin A5/Annexin V ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human Cathepsin C.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all Annexin V (ANXA5) ELISA试剂盒
- Annexin V (ANXA5) (Annexin A5 (ANXA5))
- 别名
- Annexin A5 (ANXA5 产品)
- 别名
- anx ELISA Kit, anx5 ELISA Kit, ANX V ELISA Kit, anxa5 ELISA Kit, cb989 ELISA Kit, wu:fa98f06 ELISA Kit, wu:fj10f10 ELISA Kit, MGC89158 ELISA Kit, ANX5 ELISA Kit, ENX2 ELISA Kit, PP4 ELISA Kit, RPRGL3 ELISA Kit, Anx5 ELISA Kit, R74653 ELISA Kit, LC5 ELISA Kit, enx2 ELISA Kit, annexin A5 ELISA Kit, annexin A5b ELISA Kit, Annexin A5 ELISA Kit, annexin A5 L homeolog ELISA Kit, ANXA5 ELISA Kit, anxa5b ELISA Kit, anxa5 ELISA Kit, Anxa5 ELISA Kit, anxa5.L ELISA Kit
- 基因ID
- 308
- UniProt
- P08758
- 途径
- Apoptosis
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