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INA ELISA 试剂盒
INA
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
INAELISA Kit详情
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INA
(Internexin Neuronal Intermediate Filament Protein, alpha (INA))
适用
All reactivities for INA ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Custom Human Alpha-Internexin ELISA Kit.
样品类型
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
特异性
This ELISA antibody pair recognizes Human C3a.
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
INA目标详情
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抗原
See all INA ELISA试剂盒
INA
(Internexin Neuronal Intermediate Filament Protein, alpha (INA))
别名
alpha-Internexin (INA 产品 )
别名
NEF5 ELISA Kit, NF-66 ELISA Kit, TXBP-1 ELISA Kit, Inexa ELISA Kit, Intlaa ELISA Kit, Nf66 ELISA Kit, AV028420 ELISA Kit, NF66 ELISA Kit, ina-A ELISA Kit, xefiltin ELISA Kit, ina-a ELISA Kit, inexa ELISA Kit, MGC82496 ELISA Kit, INA ELISA Kit, si:ch211-67n3.6 ELISA Kit, nef5 ELISA Kit, xina ELISA Kit, nf-66 ELISA Kit, txbp-1 ELISA Kit, MGC69277 ELISA Kit, gefiltin ELISA Kit, zgc:110516 ELISA Kit, internexin neuronal intermediate filament protein alpha ELISA Kit, internexin neuronal intermediate filament protein, alpha ELISA Kit, internexin neuronal intermediate filament protein alpha S homeolog ELISA Kit, low molecular weight neuronal intermediate filament S homeolog ELISA Kit, internexin neuronal intermediate filament protein, alpha a ELISA Kit, internexin neuronal intermediate filament protein, alpha b ELISA Kit, INA ELISA Kit, Ina ELISA Kit, ina.S ELISA Kit, nif.S ELISA Kit, inaa ELISA Kit, ina ELISA Kit, inab ELISA Kit
基因ID
9118
UniProt
Q16352
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