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AIF ELISA 试剂盒
AIFM1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
AIFELISA Kit详情
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AIF (AIFM1)
(Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))
适用
All reactivities for AIF ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Custom Human AIF ELISA Kit.
样品类型
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
特异性
This ELISA antibody pair recognizes Human B7-H4.
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
AIF目标详情
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AIF (AIFM1)
(Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))
别名
AIF (AIFM1 产品 )
别名
AIF ELISA Kit, CMTX4 ELISA Kit, COWCK ELISA Kit, COXPD6 ELISA Kit, PDCD8 ELISA Kit, CG7263 ELISA Kit, DmAIF ELISA Kit, Dmel\\CG7263 ELISA Kit, GB16024 ELISA Kit, DDBDRAFT_0187853 ELISA Kit, DDBDRAFT_0191137 ELISA Kit, DDB_0187853 ELISA Kit, DDB_0191137 ELISA Kit, aif ELISA Kit, pdcd8 ELISA Kit, AIFM1 ELISA Kit, PCD8 ELISA Kit, AIFsh2 ELISA Kit, Hq ELISA Kit, Pdcd8 ELISA Kit, mAIF ELISA Kit, Aif ELISA Kit, zgc:91994 ELISA Kit, apoptosis inducing factor mitochondria associated 1 ELISA Kit, allograft inflammatory factor 1 ELISA Kit, Apoptosis inducing factor ELISA Kit, apoptosis-inducing factor 1, mitochondrial ELISA Kit, apoptosis inducing factor ELISA Kit, apoptosis inducing factor, mitochondria associated 1 ELISA Kit, apoptosis-inducing factor, mitochondrion-associated, 1 ELISA Kit, apoptosis-inducing factor, mitochondrion-associated 1 ELISA Kit, AIFM1 ELISA Kit, AIF1 ELISA Kit, AIF ELISA Kit, LOC412212 ELISA Kit, aif ELISA Kit, aifm1 ELISA Kit, Aifm1 ELISA Kit
基因ID
9131
UniProt
O95831
途径
Apoptosis , Positive Regulation of Endopeptidase Activity , Cell RedoxHomeostasis , Smooth Muscle Cell Migration , Warburg Effect
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