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HPGD ELISA 试剂盒

HPGD 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant
产品编号 ABIN4881689
发货至: 中国
  • 抗原 See all HPGD ELISA试剂盒
    HPGD (Hydroxyprostaglandin Dehydrogenase 15-(NAD) (HPGD))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human 15-PGDH/HPGD ELISA Kit for Cell Culture Supernatants.
    样品类型
    Cell Culture Supernatant
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human 15-PGDH.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product HPGD ELISA Kit
  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all HPGD ELISA试剂盒
    HPGD (Hydroxyprostaglandin Dehydrogenase 15-(NAD) (HPGD))
    别名
    15-PGDH (HPGD 产品)
    别名
    CBR ELISA Kit, SDR21C1 ELISA Kit, hCBR1 ELISA Kit, 15-PGDH ELISA Kit, PGDH ELISA Kit, PGDH1 ELISA Kit, PHOAR1 ELISA Kit, SDR36C1 ELISA Kit, MGC80546 ELISA Kit, MGC79454 ELISA Kit, AV026552 ELISA Kit, carbonyl reductase 1 ELISA Kit, 15-hydroxyprostaglandin dehydrogenase ELISA Kit, hydroxyprostaglandin dehydrogenase 15-(NAD) ELISA Kit, 15-hydroxyprostaglandin dehydrogenase L homeolog ELISA Kit, hydroxyprostaglandin dehydrogenase 15 (NAD) ELISA Kit, CBR1 ELISA Kit, HPGD ELISA Kit, hpgd.L ELISA Kit, hpgd ELISA Kit, pgdh ELISA Kit, Hpgd ELISA Kit, PTRG_03214 ELISA Kit, VDBG_09957 ELISA Kit
    基因ID
    3248
    UniProt
    P15428
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