Galectin 3 ELISA 试剂盒
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- 抗原 See all Galectin 3 (LGALS3) ELISA试剂盒
- Galectin 3 (LGALS3)
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适用
- 犬
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 2-500 pg/mL
- 最低检测浓度
- 2 pg/mL
- 应用范围
- ELISA
- 原理
- Canine Galectin-3 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Canine Galectin-3 (LGALS3)
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Recommended Dilution for serum and plasma samples50 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all Galectin 3 (LGALS3) ELISA试剂盒
- Galectin 3 (LGALS3)
- 别名
- Galectin-3 (LGALS3 产品)
- 别名
- CBP 35 ELISA Kit, Gal-3 ELISA Kit, Galectin-3 ELISA Kit, leg3 ELISA Kit, galactoside-binding ELISA Kit, galectin-3 ELISA Kit, lectin ELISA Kit, soluble ELISA Kit, CBP35 ELISA Kit, GAL3 ELISA Kit, GALBP ELISA Kit, GALIG ELISA Kit, L31 ELISA Kit, LGALS2 ELISA Kit, MAC2 ELISA Kit, MGC52947 ELISA Kit, MGC75803 ELISA Kit, LGALS3 ELISA Kit, gal-3 ELISA Kit, GBP ELISA Kit, L-34 ELISA Kit, Mac-2 ELISA Kit, gal3 ELISA Kit, galectin 3 ELISA Kit, Galectin-3 ELISA Kit, avian beta-defensin 3 ELISA Kit, lectin, galactoside-binding, soluble, 3 L homeolog ELISA Kit, lectin, galactoside-binding, soluble, 3 ELISA Kit, galectin 2 ELISA Kit, lectin, galactose binding, soluble 3 ELISA Kit, LGALS3 ELISA Kit, leg3 ELISA Kit, AvBD3 ELISA Kit, lgals3.L ELISA Kit, lgals3 ELISA Kit, LGALS2 ELISA Kit, Lgals3 ELISA Kit
- 物质类
- Chemical
- UniProt
- P38486
- 途径
- RTK signaling
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