Chromogranin A ELISA 试剂盒
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- 抗原 See all Chromogranin A (CHGA) ELISA试剂盒
- Chromogranin A (CHGA)
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适用
- 人, 小鼠, 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 0.1-1.000 ng/mL
- 最低检测浓度
- 0.1 ng/mL
- 应用范围
- ELISA
- 原理
- Human/Mouse/Rat Catestatin EIA Kit optimized for serum, plasma and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This EIA kit is designed to detect human, mouse, and rat Catestatin.
- 灵敏度
- 0.5 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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Chromogranin A (CHGA) ELISA Kit
CHGA 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Chromogranin A (CHGA) ELISA KitCHGA 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum, Urine
Chromogranin A (CHGA) ELISA KitCHGA 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chromogranin A (CHGA) ELISA KitCHGA 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Chromogranin A (CHGA) ELISA KitCHGA 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chromogranin A (CHGA) ELISA KitCHGA 适用: 小鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Chromogranin A (CHGA) ELISA KitCHGA 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Chromogranin A (CHGA) ELISA KitCHGA 适用: 人 Colorimetric Sandwich ELISA 62.5 ng/mL - 4000 ng/mL Cell Culture Supernatant, Cerebrospinal Fluid, Plasma, Serum, Urine
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- 应用备注
- Recommended Dilution for serum and plasma samplesHuman: 12X / Mouse: 12X / Rat: 8X
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Standard, Biotinylated peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
- 有效期
- 6 months
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- 抗原 See all Chromogranin A (CHGA) ELISA试剂盒
- Chromogranin A (CHGA)
- 别名
- Catestatin (CHGA 产品)
- 别名
- CGA ELISA Kit, CG-ALPHA ELISA Kit, FSHA ELISA Kit, GPHA1 ELISA Kit, GPHa ELISA Kit, HCG ELISA Kit, LHA ELISA Kit, TSHA ELISA Kit, ChrA ELISA Kit, fj05f09 ELISA Kit, si:dkey-177p2.2 ELISA Kit, wu:fj05f09 ELISA Kit, zgc:101749 ELISA Kit, zgc:56075 ELISA Kit, CHGA ELISA Kit, cgA ELISA Kit, chga ELISA Kit, chromogranin A ELISA Kit, glycoprotein hormones, alpha polypeptide ELISA Kit, uncharacterized CHGA ELISA Kit, chromogranin A S homeolog ELISA Kit, CHGA ELISA Kit, CGA ELISA Kit, Chga ELISA Kit, chga ELISA Kit, chga.S ELISA Kit
- 基因ID
- 1113
- UniProt
- P10645
- 途径
- Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling
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