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MAP2K6 ELISA 试剂盒

MAP2K6 适用: 人, 小鼠 pSer207 Colorimetric Cell ELISA Cell Culture Cells
产品编号 ABIN4881293
发货至: 中国
  • 抗原 See all MAP2K6 ELISA试剂盒
    MAP2K6 (Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6))
    抗原表位
    pSer207
    适用
    • 5
    • 4
    • 3
    • 1
    人, 小鼠
    检测方法
    Colorimetric
    实验类型
    Cell ELISA
    应用范围
    ELISA
    原理
    Cell-Based Human/Mouse MKK6 (S207) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.
    样品类型
    Cell Culture Cells
    Analytical Method
    Semi-Quantitative
    特异性
    The antibodies provided in this kit recognizes human/mouse/rat MKK6 phosphorylated at Serine-207 and total MMK6 for comparison
    产品特性
    • Site and signal pathway-specific
    • In vitro detection of adherent cell culture
    • No sample lysis needed
    • Compatible with a standard ELISA plate reader
    • Faster results than with ELISA
    • Adaptable for high-throughput screening and drug discovery
    组件
    • uncoated 96-well Microplate
    • Wash Buffer A
    • Wash Buffer B
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    试剂未包括
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product MAP2K6 ELISA Kit
  • 样本量
    100 μL
    板类型
    Uncoated
    实验流程
    1. Seed 10,000-30,000 cells into each well and incubate overnight.
    2. Apply various treatment, inhibitors or activators according to manufacture's instructions.
    3. Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.
    4. Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.
    5. Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.
    6. Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
    7. Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
    有效期
    6 months
  • 抗原 See all MAP2K6 ELISA试剂盒
    MAP2K6 (Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6))
    别名
    MKK6 (MAP2K6 产品)
    别名
    MAPKK6 ELISA Kit, MEK6 ELISA Kit, MKK6 ELISA Kit, PRKMK6 ELISA Kit, SAPKK-3 ELISA Kit, SAPKK3 ELISA Kit, Mkk6 ELISA Kit, Prkmk6 ELISA Kit, MKK3 ELISA Kit, map2k3 ELISA Kit, zMKK3 ELISA Kit, map2k6 ELISA Kit, mitogen-activated protein kinase kinase 6 ELISA Kit, mitogen-activated protein kinase kinase 6 L homeolog ELISA Kit, MAP2K6 ELISA Kit, Map2k6 ELISA Kit, map2k6 ELISA Kit, map2k6.L ELISA Kit
    基因ID
    5608
    UniProt
    P52564
    途径
    MAPK Pathway, TLR signaling, Activation of Innate immune Response, Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades
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