AKT1 ELISA 试剂盒
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- 抗原 See all AKT1 ELISA试剂盒
- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
- 抗原表位
- pSer473
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适用
- 人, 小鼠, 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Cell ELISA
- 应用范围
- ELISA
- 原理
- Cell-Based Human/Mouse/Rat AKT (S473) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.
- 样品类型
- Cell Culture Cells
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibodies provided in this kit recognizes human/mouse/rat AKT phosphorylated at Serine-473 and total AKT for comparison.
- 产品特性
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- Site and signal pathway-specific
- In vitro detection of adherent cell culture
- No sample lysis needed
- Compatible with a standard ELISA plate reader
- Faster results than with ELISA
- Adaptable for high-throughput screening and drug discovery
- 组件
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- uncoated 96-well Microplate
- Wash Buffer A
- Wash Buffer B
- Fixing Solution
- Quenching Buffer
- Blocking Buffer
- Anti-phospho antibody
- Anti-pan antibody
- HRP-Conjugated Secondary Antibody
- TMB One-Step Substrate
- Stop Solution
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Featured
- Discover our best selling AKT1 ELISA Kit
- Top Product
- Discover our top product AKT1 ELISA Kit
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V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA Kit
AKT1 适用: 人, 小鼠, 大鼠 pSer473 Colorimetric Sandwich ELISA Cell Lysate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 人, 小鼠, 大鼠 pSer473 Colorimetric Sandwich ELISA Cell Lysate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 小鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Uncoated
- 实验流程
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- Seed 10,000-30,000 cells into each well and incubate overnight.
- Apply various treatment, inhibitors or activators according to manufacture's instructions.
- Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.
- Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.
- Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.
- Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
- Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
- 有效期
- 6 months
-
- 抗原 See all AKT1 ELISA试剂盒
- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
- 别名
- Akt (AKT1 产品)
- 别名
- AKT ELISA Kit, CWS6 ELISA Kit, PKB ELISA Kit, PKB-ALPHA ELISA Kit, PRKBA ELISA Kit, RAC ELISA Kit, RAC-ALPHA ELISA Kit, Akt ELISA Kit, Rac ELISA Kit, PKB/Akt ELISA Kit, PKBalpha ELISA Kit, AKT/PKB ELISA Kit, AKT1 ELISA Kit, Akt/PKB ELISA Kit, Akt1 ELISA Kit, CG4006 ELISA Kit, D-Akt ELISA Kit, DAKT1 ELISA Kit, DAKT1/PKB ELISA Kit, DAkt ELISA Kit, DAkt1 ELISA Kit, DPKB ELISA Kit, DRAC-PK ELISA Kit, DRAC-PK66 ELISA Kit, DRAC-PK85 ELISA Kit, Dakt ELISA Kit, Dakt1 ELISA Kit, Dmel\\CG4006 ELISA Kit, Dpkb ELISA Kit, PKB/AKT ELISA Kit, PKB/dAKT ELISA Kit, RacPK ELISA Kit, akt ELISA Kit, akt1 ELISA Kit, dAKT ELISA Kit, dAKT/dPKB ELISA Kit, dAKT1 ELISA Kit, dAkt ELISA Kit, dAkt/PKB ELISA Kit, dAkt1 ELISA Kit, dPKB ELISA Kit, dakt ELISA Kit, dakt1 ELISA Kit, l(3)04226 ELISA Kit, l(3)89Bq ELISA Kit, p-Akt ELISA Kit, pAkt ELISA Kit, ACT-5 ELISA Kit, akt-1 ELISA Kit, pkb ELISA Kit, v-akt ELISA Kit, v-akt1 ELISA Kit, xAct ELISA Kit, ATAKT1 ELISA Kit, F18A8.2 ELISA Kit, F18A8_2 ELISA Kit, K+ transporter 1 ELISA Kit, POTASSIUM TRANSPORTER ELISA Kit, AKT serine/threonine kinase 1 ELISA Kit, thymoma viral proto-oncogene 1 ELISA Kit, CG4006 gene product from transcript CG4006-RE ELISA Kit, actin beta ELISA Kit, v-akt murine thymoma viral oncogene homolog 1 S homeolog ELISA Kit, v-akt murine thymoma viral oncogene homolog 1 ELISA Kit, K+ transporter 1 ELISA Kit, Serine/threonine-protein kinase akt-1 ELISA Kit, AKT1 ELISA Kit, Akt1 ELISA Kit, ACTB ELISA Kit, akt1.S ELISA Kit, akt1 ELISA Kit, KT1 ELISA Kit, akt-1 ELISA Kit
- 基因ID
- 207
- UniProt
- P31749
- 途径
- PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect
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