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CRABP2 ELISA 试剂盒

CRABP2 适用: 人 Colorimetric Sandwich ELISA 0.312-20 ng/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN455486
发货至: 中国
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    CRABP2 (Cellular Retinoic Acid Binding Protein 2 (CRABP2))
    适用
    • 3
    • 2
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.312-20 ng/mL
    最低检测浓度
    0.312 ng/mL
    应用范围
    ELISA
    原理
    This immunoassay kit allows for the in vitro quantitative determination of human IFN?-1b concentrations in cell culture supernates, serum, plasma and other biological fluids.
    样品类型
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    This assay recognizes recombinant and natural human IFN?-1b.
    交叉反应 (详细)
    No significant cross-reactivity or interference was observed.
    灵敏度
    < 3.9 pg/mL
    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
    产品特性
    Homo sapiens,Human,Cellular retinoic acid-binding protein 2,Cellular retinoic acid-binding protein II,CRABP-II,CRABP2
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFNα-1b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IFNα-1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IFNα-1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of IFNα-1b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    限制
    仅限研究用
  • 储存条件
    4 °C/-20 °C
    储存方法
    The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
  • 抗原 See all CRABP2 ELISA试剂盒
    CRABP2 (Cellular Retinoic Acid Binding Protein 2 (CRABP2))
    别名
    CRABP2 (CRABP2 产品)
    别名
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