Haptoglobin ELISA 试剂盒
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- 抗原 See all Haptoglobin (HP) ELISA试剂盒
- Haptoglobin (HP)
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适用
- Cow
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.78-50 μg/mL
- 最低检测浓度
- 0.78 μg/mL
- 应用范围
- ELISA
- 原理
- This immunoassay kit allows for the in vitro quantitative determination of bovine Hpt concentrations in serum, plasma and other biological fluids.
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay recognizes recombinant and natural bovine Hpt.
- 交叉反应 (详细)
- No significant cross-reactivity or interference was observed.
- 灵敏度
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< 0.195 μg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero. - 产品特性
- Bos taurus,Bovine,Haptoglobin,HP,Zonulin
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- Discover our best selling HP ELISA Kit
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- Discover our top product HP ELISA Kit
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hpt. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Hpt. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hpt, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Hpt in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
- 储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- 抗原 See all Haptoglobin (HP) ELISA试剂盒
- Haptoglobin (HP)
- 别名
- HP (HP 产品)
- 别名
- HP ELISA Kit, wu:fb64e01 ELISA Kit, BP ELISA Kit, HP2ALPHA2 ELISA Kit, HPA1S ELISA Kit, HP-1 ELISA Kit, HPR ELISA Kit, Zonulin ELISA Kit, haptoglobin ELISA Kit, haptoglobin-like ELISA Kit, HP ELISA Kit, hp ELISA Kit, Hp ELISA Kit, LOC479668 ELISA Kit, LOC101102413 ELISA Kit
- 背景
- Haptoglobin (Hpt) is a plasma protein with hemoglobin-binding capacity, and a plasma glycoproteins that form a stable complex with hemoglobin to aid the recycling of heme iron. It is a well-known marker of hemolysis. High haptoglobin level in plasma was associated with an increased cardiovascular risk in obese men, inflammation, atherosclerosis, and systemic sclerosis.
- 途径
- Transition Metal Ion Homeostasis
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