Fibronectin 1 ELISA 试剂盒
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- 抗原 See all Fibronectin 1 (FN1) ELISA试剂盒
- Fibronectin 1 (FN1)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.78-50 ng/mL
- 最低检测浓度
- 0.78 ng/mL
- 应用范围
- ELISA
- 原理
- This immunoassay kit allows for the in vitro quantitative determination of mouse Fibronectin,FN concentrations in cell culture supernates, serum, plasma and other biological fluids.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay recognizes recombinant and natural mouse FN.
- 交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
- 灵敏度
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< 11.5 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero. - 产品特性
- Mus musculus,Mouse,Fibronectin,FN,Fn1
- Featured
- Discover our best selling FN1 ELISA Kit
- Top Product
- Discover our top product FN1 ELISA Kit
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Fibronectin 1 (FN1) ELISA Kit
FN1 适用: 人 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibronectin 1 (FN1) ELISA KitFN1 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Fibronectin 1 (FN1) ELISA KitFN1 适用: 小鼠 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Fibronectin 1 (FN1) ELISA KitFN1 适用: 人 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Fibronectin 1 (FN1) ELISA KitFN1 适用: 大鼠 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Fibronectin 1 (FN1) ELISA KitFN1 适用: 兔 Colorimetric Competition ELISA 1.25 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Fibronectin 1 (FN1) ELISA KitFN1 适用: 兔 Colorimetric Sandwich ELISA 1.25-20 ng/mL Plasma, Serum, Tissue Homogenate
Fibronectin 1 (FN1) ELISA KitFN1 适用: Cow Colorimetric Sandwich ELISA 0.137-100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to FN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
- 储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
-
- 抗原 See all Fibronectin 1 (FN1) ELISA试剂盒
- Fibronectin 1 (FN1)
- 别名
- Fn1 (FN1 产品)
- 途径
- Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy
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