HMGB4 ELISA 试剂盒
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- 抗原 See all HMGB4 products
- HMGB4 (High Mobility Group Box 4 (HMGB4))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156-10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 原理
- This immunoassay kit allows for the in vitro quantitative determination of human chondroitin sulfatase,CS concentrations in cell culture supernates, serum, plasma and other biological fluids.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay recognizes recombinant and natural human CS.
- 交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
- 灵敏度
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< 0.312 U/L
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero. - 产品特性
- Homo sapiens,Human,High mobility group protein B4,HMGB4
- Featured
- Discover our best selling HMGB4 ELISA Kit
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High Mobility Group Box 4 (HMGB4) ELISA Kit
HMGB4 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
High Mobility Group Box 4 (HMGB4) ELISA KitHMGB4 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to CS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
- 储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- 抗原 See all HMGB4 products
- HMGB4 (High Mobility Group Box 4 (HMGB4))
- 别名
- HMGB4 (HMGB4 产品)
- 别名
- HMGB4 ELISA Kit, dJ1007G16.5 ELISA Kit, 1700001F22Rik ELISA Kit, AI326135 ELISA Kit, high mobility group box 4 ELISA Kit, high-mobility group box 4 ELISA Kit, HMGB4 ELISA Kit, Hmgb4 ELISA Kit
- 背景
- In enzymology, a chondroitin sulfatase is an enzyme that catalyzes the chemical reaction of cleaving off the 6-sulfate groups of the N-acetyl-D-galactosamine 6-sulfate units of the macromolecule chondroitin sulfate and, similarly, of the D-galactose 6-sulfate units of the macromolecule keratan sulfate.This enzyme belongs to the family of hydrolases, specifically those acting on sulfuric ester bonds.
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