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HMGB4 ELISA 试剂盒

HMGB4 适用: 人 Colorimetric Sandwich ELISA 0.156-10 ng/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN454716
发货至: 中国
  • 抗原 See all HMGB4 products
    HMGB4 (High Mobility Group Box 4 (HMGB4))
    适用
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.156-10 ng/mL
    最低检测浓度
    0.156 ng/mL
    应用范围
    ELISA
    原理
    This immunoassay kit allows for the in vitro quantitative determination of human chondroitin sulfatase,CS concentrations in cell culture supernates, serum, plasma and other biological fluids.
    样品类型
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    This assay recognizes recombinant and natural human CS.
    交叉反应 (详细)
    No significant cross-reactivity or interference was observed.
    灵敏度
    < 0.312 U/L
    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
    产品特性
    Homo sapiens,Human,High mobility group protein B4,HMGB4
    Featured
    Discover our best selling HMGB4 ELISA Kit
  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    The microtiter plate provided in this kit has been pre-coated with an antibody specific to CS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    限制
    仅限研究用
  • 储存条件
    4 °C/-20 °C
    储存方法
    The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
  • 抗原 See all HMGB4 products
    HMGB4 (High Mobility Group Box 4 (HMGB4))
    别名
    HMGB4 (HMGB4 产品)
    别名
    HMGB4 ELISA Kit, dJ1007G16.5 ELISA Kit, 1700001F22Rik ELISA Kit, AI326135 ELISA Kit, high mobility group box 4 ELISA Kit, high-mobility group box 4 ELISA Kit, HMGB4 ELISA Kit, Hmgb4 ELISA Kit
    背景
    In enzymology, a chondroitin sulfatase is an enzyme that catalyzes the chemical reaction of cleaving off the 6-sulfate groups of the N-acetyl-D-galactosamine 6-sulfate units of the macromolecule chondroitin sulfate and, similarly, of the D-galactose 6-sulfate units of the macromolecule keratan sulfate.This enzyme belongs to the family of hydrolases, specifically those acting on sulfuric ester bonds.
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