IgM ELISA 试剂盒
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- 抗原 See all IgM ELISA试剂盒
- IgM
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 最低检测浓度
- 312.5 ng/mL
- 应用范围
- ELISA
- 原理
- The IgM(rat)-ELISA is designed for the quantitative determination of rat IgM in complex samples (serum or other biological samples).
- 样品类型
- Serum
- Analytical Method
- Quantitative
- 产品特性
- The determination of rat IgM is carried out as direct sandwich ELISA.
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- Discover our top product IgM ELISA Kit
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- 板类型
- Pre-coated
- 实验流程
- An antibody specific for rat IgM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgM present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgM concentration.
- 实验流程
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Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm - 实验精密度
- Intraassay precision (CV):(n=10)at 70 ng/ml: 7.6%at 250 ng/ml: 6.8%
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原 See all IgM ELISA试剂盒
- IgM
- Abstract
- IgM 产品
- 别名
- IgM constant region ELISA Kit, IGM ELISA Kit
- 物质类
- Antibody
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