IgA ELISA 试剂盒
-
- 抗原 See all IgA ELISA试剂盒
- IgA
-
适用
- Pig
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- The IgA(pig)-ELISA is designed for the quantitative determination of pig IgA in complex samples (serum or other biological samples).
- 样品类型
- Serum
- Analytical Method
- Quantitative
- 产品特性
- The determination of pig IgA is carried out as direct sandwich ELISA.
- Featured
- Discover our best selling IgA ELISA Kit
- Top Product
- Discover our top product IgA ELISA Kit
-
-
- 板类型
- Pre-coated
- 实验流程
- An antibody specific for pig IgA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgA present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgA concentration.
- 实验流程
-
Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm - 实验精密度
- Intraassay precision (CV):(n=10)at 25 ng/ml: 6.6%at 100 ng/ml: 4.3%at 237 ng/ml: 7.9%
- 限制
- 仅限研究用
-
- 储存条件
- 4 °C
-
- 抗原 See all IgA ELISA试剂盒
- IgA
- Abstract
- IgA 产品
- 别名
- MB-1 ELISA Kit, IG-alpha ELISA Kit, IGA ELISA Kit, IgA ELISA Kit, Igh-2 ELISA Kit, CD79a molecule ELISA Kit, immunoglobulin heavy constant alpha ELISA Kit, CD79A ELISA Kit, Igha ELISA Kit
- 物质类
- Antibody
-