电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

Myeloperoxidase ELISA 试剂盒

MPO 适用: 人 AA 49-745 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Tissue Homogenate, Urine
产品编号 ABIN411400
发货至: 中国
  • 抗原 See all Myeloperoxidase (MPO) ELISA试剂盒
    Myeloperoxidase (MPO)
    抗原表位
    AA 49-745
    适用
    • 13
    • 10
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    312-20000 pg/mL
    最低检测浓度
    312 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MPO
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: NSO
    Immunogen sequence: A49-S745
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <10pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: NSO
    Immunogen sequence: A49-S745
    Featured
    Discover our best selling MPO ELISA Kit
    Top Product
    Discover our top product MPO ELISA Kit
  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Sequence similarities: Belongs to the peroxidase family. XPO subfamily.

    板类型
    Pre-coated
    实验流程
    human MPO ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MPO has been precoated onto 96-well plates. Standards(NSO, A49-S745) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MPO amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human MPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysate, tissue lysate, serum, plasma(heparin, EDTA), saliva or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MPO standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(ng/ml): 2.3, Standard deviation: 0.076, CV(%): 3.3
    • Sample 2: n=16, Mean(ng/ml): 7.4, Standard deviation: 0.4, CV(%): 5.4
    • Sample 3: n=16, Mean(ng/ml): 12.4, Standard deviation: 0.72, CV(%): 5.8,
    • Sample 1: n=24, Mean(ng/ml): 2.9, Standard deviation: 0.122, CV(%): 4.2
    • Sample 2: n=24, Mean(ng/ml): 8, Standard deviation: 0.488, CV(%): 6.1
    • Sample 3: n=24, Mean(ng/ml): 14.7, Standard deviation: 0.956, CV(%): 6.5
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • Fu, Li, Sun, Ma, Yao: "Bradykinin inhibits oxidative stress-induced senescence of endothelial progenitor cells through the B2R/AKT/RB and B2R/EGFR/RB signal pathways." in: Oncotarget, Vol. 6, Issue 28, pp. 24675-89, (2016) (PubMed).

    Liu, Chen, Wu, Chen, Zhang: "Neutrophil serine proteases and their endogenous inhibitors in coronary artery ectasia patients." in: Anatolian journal of cardiology, Vol. 16, Issue 1, pp. 23-8, (2016) (PubMed).

    Sousa, Morad, Feliciano, Pita, Nady, El-Hennamy, Abdel-Rahman, Cavaco, Pereira, Barreto, Leitão: "The Burkholderia cenocepacia OmpA-like protein BCAL2958: identification, characterization, and detection of anti-BCAL2958 antibodies in serum from B. cepacia complex-infected Cystic Fibrosis patients." in: AMB Express, Vol. 6, Issue 1, pp. 41, (2016) (PubMed).

    Chen, Yang, Liu, Teng, Ye, Xu, Cai, Cheng, Yang, Hu, Wang, Cao: "Banxia xiexin decoction protects against dextran sulfate sodium-induced chronic ulcerative colitis in mice." in: Journal of ethnopharmacology, Vol. 166, pp. 149-56, (2015) (PubMed).

    Wang, Tang, Li, Wang: "Biochanin A protects against focal cerebral ischemia/reperfusion in rats via inhibition of p38-mediated inflammatory responses." in: Journal of the neurological sciences, Vol. 348, Issue 1-2, pp. 121-5, (2015) (PubMed).

    Wu, Ming, Zhang, Wu, Wu, Yao: "Edaravone rescues the lung by inhibiting lipid peroxidation and pro-inflammatory cytokines in a rat model." in: Chinese medical journal, Vol. 127, Issue 5, pp. 968-70, (2014) (PubMed).

    Chen, Chen, Qin: "Effects of polysaccharides of the Euphoria Longan (Lour.) Steud on focal cerebral ischemia/reperfusion injury and its underlying mechanism." in: Brain injury, Vol. 25, Issue 3, pp. 292-9, (2011) (PubMed).

  • 抗原 See all Myeloperoxidase (MPO) ELISA试剂盒
    Myeloperoxidase (MPO)
    别名
    MPO (MPO 产品)
    别名
    mKIAA4033 ELISA Kit, MPO ELISA Kit, LOC100335032 ELISA Kit, POX2' ELISA Kit, XPOX2' ELISA Kit, mpo ELISA Kit, mpo-A ELISA Kit, pmr-1 ELISA Kit, pmr1 ELISA Kit, pox2 ELISA Kit, xpox2 ELISA Kit, myeloperoxidase ELISA Kit, myeloperoxidase L homeolog ELISA Kit, MPO ELISA Kit, Mpo ELISA Kit, LOC100335032 ELISA Kit, mpo.L ELISA Kit
    背景

    Protein Function: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

    Background: Myeloperoxidase(MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function. The standard product used in this kit is the product of gene recombination, consisting of 697(A49-S745) amino acids with the molecular mass of 80KDa.

    Synonyms: Myeloperoxidase,MPO,1.11.2.2,Myeloperoxidase,89 kDa myeloperoxidase,84 kDa myeloperoxidase,Myeloperoxidase light chain,Myeloperoxidase heavy chain,MPO,

    Full Gene Name: Myeloperoxidase

    Cellular Localisation: Lysosome.
    基因ID
    4353
    UniProt
    P05164
    途径
    Chromatin Binding
You are here:
客服